| 產(chǎn)品名稱 |
pCRII-mp27 |
| 商品貨號(hào) |
B212636 |
| Designations |
pCRII-mp27 |
| GenBank Number |
U09968
|
| Species |
Mus musculus, mouse |
| Depositors |
SJ Elledge |
| Vector |
Construct size (kb): 4.5 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.6110000014305115 Tissue: cDNA Gene product: cyclin-dependent kinase inhibitor (p27)(Cyclin-dependent kinase inhibitor 1B (p27, Kip1)) [Kip1] |
| Insert Size (kb) |
0.611 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BamHI/NdeI--4.1, 0.66; EcoRI--4.1, 0.70; PstI--3.0, 1.25, 0.43, 0.25; SmaI--4.8. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.09, 0.47; SmaI--0.13. Constructed by amplifying the coding sequence by PCR with primers that that introduced the NdeI and BamHI sites. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Matsuoka S, et al. p57KIP2, a structurally distinct member of the p21CIP1 Cdk-inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9: 650-662, 1995. PubMed: 7729684
Stephen J Elledge, personal communication
|
