Expression vector regulated by nalidixic acid induction of the recA promoter. The 236 bp EcoRI/BamHI fragment can serve as a portable promoter.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Shirakawa M, et al. Plasmid vectors designed for high-efficiency expression controlled by the portable recA promoter-operator of Escherichia coli. Gene 28: 127-132, 1984. PubMed: 6329913
