| 產品名稱 |
Canine parvovirus |
| 商品貨號 |
B212795 |
| Classification |
Parvoviridae, Parvovirus, Feline panleukopenia virus |
| Agent |
Canine parvovirus |
| Strain |
Cornell-780916-80 |
| Applications |
Efficacy testing |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
freeze-dried |
| Storage Conditions |
?-70°C or colder? |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
Reduced virulence variant obtained by passage at 33°C. For best titer and CPE, infect actively growing cells. Virus agglutinates pig and rhesus monkey erythrocytes. |
| Effect on Host |
CPE; refractile cell rounding and sloughing |
| Recommended Host |
A-72 (ATCC® CRL-1542™) |
| Growth Conditions |
Temperature: 37°C
Incubation: Incubate infected A-72 cell culture for 3-5 days at 37°C in 100% atmospheric air, until CPE is well advanced through 90% of the culture.
Recommendations for Infection: Plant cells 6 hours in advance and infect when cultures are 40-50% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2
) of virus diluted with virus growth medium to provide a MOI of about 1.0. Adsorb 1 hour at 37°C in 100% atmospheric air in nonvented flasks. End adsorption by adding viral growth media. |
| Name of Depositor |
Cornell Res. Fndn., Inc. |
| Source |
Intestinal contents of beagle pups with severe enteritis |
| References |
Carmichael LE, et al. Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am. J. Vet. Res. 40: 784-791, 1980. PubMed: 6250432
Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.
|
