Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
mutant derived from ATCC 40335
Product Format
frozen
Type Strain
no
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Medium
ATCC® Medium 2085: YEP medium
Growth Conditions
Temperature: 30.0°C
Duration: axenic
Protocol: This strain is routinely shipped as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the entire contents to 5 ml of ATCC medium 200 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen it by one half turn, and incubate the tube on a horizontal slant (5-15 degree slant) at 30C. The culture should be ready to subculture in 5-7 days. To subculture, crew the cap on tightly, agitate the tube vigorously, and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2085. Incubate as above.
Subcultivation
Protocol: This strain is routinely shipped as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the entire contents to 5 ml of ATCC medium 200 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen it by one half turn, and incubate the tube on a horizontal slant (5-15 degree slant) at 30C. The culture should be ready to subculture in 5-7 days. To subculture, crew the cap on tightly, agitate the tube vigorously, and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2085. Incubate as above.
Name of Depositor
Coors BioTech, Inc., SA Orndorff, Coors BioTech, Inc.
