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Size (kb): 6.2280001640319820

Vector: pCu424CTR1-CYC1 (phagemid)

Promoters: Promoter for expression CTR1-CYC1

Construction: pRS424, CTR1-CYC1 promoter

Marker(s):TRP1,ampR

Construct size (kb): 6.228000164031982

Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1

cloning vector

Restriction digests of the vector give the following sizes (kb): SacI--6.2; SacI/SpeI--5.9, 0.4.

CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.

component of:ATCC 87778