人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

Size (kb): 7.0000000000000000

Vector: YIp365 (plasmid)

Promoters: Promoter none

Construction: YEp365 (ATCC 37730), YIp351

Marker(s):LEU2,ampR

Construct size (kb): 7.0

Features: insert detection: lacZ
marker(s): ampR, LEU2
promoter: none
replicon: pMB1

YI-type (integrating) shuttle vector

promoter-cloning vector

shuttle vector

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SmaI-1; BamHI-1; SalI-1; PstI-2; HindIII-3. The EcoRI site is not unique.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.9; SalI--7.0; PstI--7.0; HindIII--7.0.

One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37746 - 37748) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5' G AAT TCC CGG GGA TCC GTC GAC CTG CAG CCA AGC TTC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.

Temperature: 37.0°C