| 產品名稱 |
HHCPN85 |
| 商品貨號 |
B213128 |
| Designations |
HHCPN85 |
| GenBank Number |
M78165
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.599999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CALM3
Other gene symbols: PHKD
Gene name: calmodulin 3
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.3
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M78165; EST: EST01758; Primer: M13 Forward;
THC id: THC171455
DNA Seq. Acc.: M86171; EST: EST02703; Primer: M13 Reverse;
THC id: THC171455 Insert lengths(kb): 1.700000047683716 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: calmodulin 3 [PHKD] |
| Insert Size (kb) |
1.700 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.0, 1.5. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.7; BamHI--4.6; PstI--3.2, 1.0, 0.25. The gene may actually be calmodulin 1, not calmodulin 3. EST sequences are too short to determine which it is. EST02703 matches GenBank accession HUMCAMA with 96.06% identity over the sequence range: 161-313. EST01758 matches GenBank accession HUMCAMA with 96.45% identity over the sequence range: 311-1. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
|
