Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
ATCC® 50154™
Product Format
frozen
Storage Conditions
Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures: 2-8°C
Live Cultures: See Protocols section for handling information
Type Strain
no
Genome Sequenced Strain
Yes
Comments
Genome strain developed from ATCC 50154
Contains Flavobacterium sp. as single bacterial food source
Genome sequencing strain (DOE)
Medium
ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 25°C
Cryopreservation
Reagents Cryoprotective Solution
DMSO: 2.0 mL
Fresh growth medium w/o bacteria: 8.0 mL
Harvesting and Preservation
Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.
Adjust the concentration of cells at least 2 x 106/mL in fresh medium.
Mix the cell preparation and the cryoprotective solution in equal portions.
Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
To establish a culture from the frozen state place the vial in a 35°C water bath. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 mL uninoculated ATCC medium 1525.
Incubate at 25°C with the cap screwed on tightly.
Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 mL to 10.0 mL of uninoculated ATCC medium 1525.
Follow the protocol for maintenance of culture.
Name of Depositor
Nicole King
References
Abedin M and King N. The premetazoan ancestry of cadherins. Science 319:946-948, 2008. PubMed:18276888
King N, et al. The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature 451:783-788, 2008. PubMed:18273011
