| Vector |
Construct size (kb): 4.80
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references: |
| Insert |
DNA: cDNA
Genome: human
Gene symbol: TNFA
Genomic copy number: unique
Gene name: tumor necrosis factor, alpha (cachectin)
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 p21.3
Tissue: HL-60 cell line (promyelocytic leukemia)
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 1.585
Cross references: DNA Seq. Acc.: M10988
Insert lengths(kb): 1.59
Tissue: HL-60 cell line (promyelocytic leukemia)
Gene product: tumor necrosis factor, alpha (cachectin) [TNFA] |
| Comments |
The insert contains recognition sites for the following enzymes: HgiA1 (2), BglI (2), NarI, AvaI, PvuII (2), HindIII, EcoRI, PstI, StuI (2). A PstI digest will release a 1.1 kb fragment which contains the TNF coding sequence plus some vector sequence at the 5' end and some 3' untranslated sequence. Restriction digests of the clone give the following sizes (kb): PstI--2.4, 1.4, 1.1; HindIII--3.4, 1.5; BamHI--3.1, 1.8; EcoRI--4.6; EcoRI/PstI--2.4, 1.5, 1.0; EcoRI/BamHI--3.1, 1.1, 0.7; HindIII/PstI--2.4, 0.9, 0.8, 0.5; BamHI/HindIII--2.7, 1.0, 0.74, 0.47; StuI--3.5, 2.2, 0.25. |
| References |
Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,063 dated Jun 30 1987
Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,064 dated Jun 30 1987
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