| 產品名稱 |
Vaccinia virus |
| 商品貨號 |
B213844 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus |
| Strain |
vP-53 [strain VP53] |
| Applications |
produces protein hemagglutinin, serotype H1 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
New York State Department of Health strain |
| Product Format |
frozen |
| Storage Conditions |
-70°C |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
A recombinant of vaccinia (VTK-79), that contains an influenza HA gene. This clone expresses the HA gene more strongly than either vP-9 (ATCC VR-2043) or vP-10 (ATCC VR-2044). This clone expresses the hemagglutinin gene (serotype H1) of influenza virus and can be used in the production of antibodies against this protein. A HindIII fragment coding for the influenza HA gene (blunted, BamHI linkers added) was cloned into the BamHI site of a PstI subfragment of the vaccinia HindIII F-fragment. This construct was cloned into VTK-79 (ATCC VR-2031) by in vivo recombination. |
| Effect on Host |
Cytopathic effects in tissue culture |
| Name of Depositor |
E Paoletti |
| U.S. Patent |
|
| U.S. Patent Number |
|
| Source |
New York State Department of Health strain |
| References |
Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986
Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988
Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520
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