| Vector Information |
Size (kb): 4.354 Vector: pFL39 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):TRP1,ampR Construct size (kb): 4.354 Features: insert detection: lacZ'
marker(s): ampR, TRP1
promoter: lac
replicon: pMB1, ARS-CEN6
centromere: CEN6 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; BglII--3.7, 0.85; ClaI--3.75, 0.8. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 11.0. YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR. |
| References |
Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
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