Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Macaca nemestina, West Malaysia, 1964
Product Format
frozen
Type Strain
no
Comments
Susceptibility of some Malaysian mosquitoes to infection
This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor
National Institutes of Health
Year of Origin
1964
References
Bennett GF, et al. Biology of the simian malarias of southeast Asia. II. The susceptibility of some Malaysian mosquitoes to infection with five strains of Plasmodium cynomolgi. J. Parasitol. 52: 625-631, 1966. PubMed: 5969101
Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.
