| Vector Information |
Size (kb): 9.0640001296997070 Vector: pMMB190 (plasmid) Promoters: Promoter tac Construction: pMMB66EH, pUC8 Marker(s):ampR Construct size (kb): 9.064000129699707 Features: insert detection: lacZ'
marker(s): ampR
promoter: tac, lacUV5
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0; EcoRI/MluI--8.2, 0.9. Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction. |
