| 產品名稱 |
Human rhinovirus 49 |
| 商品貨號 |
B214234 |
| Classification |
Picornaviridae, Rhinovirus |
| Agent |
Human rhinovirus 49 |
| Strain |
8213 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1). |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Genome Sequenced Strain |
Yes
|
| Comments |
This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1). |
| Effect on Host |
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells. |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
|
| Recommended Host |
Production Host:H1-HeLa (ATCC CRL-1958) Production Host: H1-HeLa (ATCC? CRL-1958™) Alternate Hosts:WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells. |
| Growth Conditions |
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidly as possible. |
| Name of Depositor |
NIAID, ATCC |
| Source |
Presumed from human throat washings; derived from ATCC VR-1159 following treatment to remove mycoplasma contamination. |
| References |
Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.
Kistler AL, et al. Genome-wide diversity and selective pressure in the human rhinovirus. Virol. J. 4: 40, 2007. PubMed: 17477878
|
