| Comments |
Isolate plasmids from mid-log phase cells following the protocol in Biochemistry 9: 4428-4439, 1970 exactly. DNA preparations are best stored at -20C divided into small samples. natural isolate May be used as covalently-closed circular markers in agarose gel electrophoresis. This strain contains 8 plasmids: A--53.23 kb, B--7.16 kb, C--5.45 kb, D--5.04 kb, E--3.91 kb, F--3.02 kb, G--2.66 kb, H--2.02 kb. |
