| 產(chǎn)品名稱 |
HFBCG24 |
| 商品貨號(hào) |
B214330 |
| Designations |
HFBCG24 |
| GenBank Number |
M77976
T04940
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ZNF41
Gene name: zinc finger protein 41
Contains complete coding sequence?: U
Chromosome: X; Localization: X p11.3-p11.23
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: DXS1004E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: X; Localization: X p11.3-p11.23
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.35
Cross references: DNA Seq. Acc.: M77976; EST: EST01560; Primer: M13 Forward
DNA Seq. Acc.: T04940; EST: EST02828; Primer: M13 Reverse;
THC id: THC100628 Insert lengths(kb): 2.349999904632568 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: DNA Segment, single copy, expressed [ZNF41] |
| Insert Size (kb) |
2.350 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.6, 0.5, 0.25. EST01560 matches GenBank accession M92443 with 97% identity over a length of 130 nt. EST02828 does not show a significant match to any nucleotide sequence record at this time. In particular, it does not match GenBank M92443 for zinc finger protein ZNF41. EST01560 matches PIR accession S00754 with 66.98% identity and 77.34% similarity over the sequence range: 17-334. This sequence record is for human zinc finger protein (HF.12). |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
Polymeropoulos MH, et al. Chromosomal distribution of 320 genes from a brain cDNA library. Nat. Genet. 4: 381-386, 1993. PubMed: 8401586
Parrish JE, Nelson DL. Regional assignment of 19 X-linked ESTs. Hum. Mol. Genet. 2: 1901-1905, 1993. PubMed: 8281154
J C Venter, personal communication
|
