| 產(chǎn)品名稱 | PEAKrapid |
|---|---|
| 商品貨號 | B214338 |
| Organism | Homo sapiens, human |
| Tissue | kidney |
| Cell Type | transformed with adenovirus 5 DNA |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | loosely adherent |
| Biosafety Level | 2 [cells containing Adenovirus type 5 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | fetus |
| Applications | The cells are useful for protein expression. They support the replication of recombinant plasmids with the Epstein-Barr virus (EBV) oriP or SV40 origin of replication. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | This line was derived from the human embryonic kidney line, HEK 293, ATCC CRL-1573. |
| Complete Growth Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.05 mg/ml Gentamicin sulfate and 10% fetal bovine serum.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Interval: Maintain cultures at a cell concentration between 8 x 103 and 1 x 105 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every two to three days
Note:For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation | Complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C |
| Population Doubling Time | 26 hours |
| Name of Depositor | J Seed |
| References | Nelson G, et al. An amino-acid taste receptor. Nature 416: 199-202, 2002. PubMed: 11894099 Magistrelli G, et al. Rapid, simple and high yield production of recombinant proteins in mammalian cells using a versatile episomal system. Protein Expression and Purification: 72(2): 209-216, 2010. PubMed: 20399863 Dobi A, et al. Mammalian expression cloning of nucleic acid binding proteins by agarose thin-layer gelshift clone selection. Biotechniques: 33(4): 868-872, 2002. PubMed: 12398195 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
