IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A broad host range cosmid vector. It was constructed by replacing the 0.75 kb PstI fragment of pMMB7 by the 5.7 kb PstI fragment of pMMB31, which contains the lambda-cos site and the Km-resistance gene. It differs from pMMB33, ATCC# 37248, only by the orientation of the 5.7 kb fragment. |
