| 產品名稱 |
pKB750 |
| 商品貨號 |
B214833 |
| Designations |
pKB750 |
| GenBank Number |
M10431
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
OmniGene, Inc., KC Backman, OmniGene, Inc. |
| Applications |
produces protein chorismate mutase-P-prephenate dehydrogenase |
| Vector |
Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic Insert lengths(kb): 1.379999995231628 Gene product: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase [aroF] Target Gene: chorismate mutase-P-prephenate dehydrogenase |
| Insert Size (kb) |
1.380 |
| Media |
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/KpnI--4.4, 1.4, 1.2; EcoRV--4.4, 2.6; EcoRI--4.4, 2.6. Expression from the aroF promoter results in an aroF-pheA fusion protein. Insert contains aa 1-351 of the aroF coding sequence and the complete pheA coding sequence. The construct contains a weak attenuator sequence at the 5' end of the pheA coding sequence. Production of phenylalanine is enhanced when the pheA structural gene is introduced as part of a synthetic operon, rather than as a single deregulated structural gene. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989
Backman KC. Enzyme deregulation. US Patent 4,753,883 dated Jun 28 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
