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Size (kb): 7.1999998092651370

Vector: pADbeta (plasmid)

Promoters: Promoter Ad2 major late

Construction: pUC19, pC4AUG beta-gal, p91023(B)

Marker(s):ampR

Construct size (kb): 7.199999809265137

Features: insert detection: lacZ'
marker(s): ampR
promoter: Ad2 major late
replicon: pMB1
terminator: SV40 late

contains easily purifiable cassette(s) for construction

reporter construct

vector for other uses

vector permitting visual detection of activity in colonies

Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--3.9, 3.3; EcoRI/HindIII--3.9, 2.7, 0.6; NotI--3.6 (doublet); BamHI--7.0, 0.2.

Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.

Designed primarily as an enhancer trap vector. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.

The lacZ gene may be excised by NotI and replaced with alternative sequences.

The order of the major features in this plasmid is: pUC19 - EcoRI - adenovirus 2 major late promoter/tripartite leader/splice signal - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19.

Temperature: 37.0°C