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LL18NS01
LL18NS01
規格:
貨期:
編號:B214926
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 LL18NS01
商品貨號 B214926
Designations LL18NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
skin fibroblast 761 cell line
Vector
Tissue: skin fibroblast 761 cell line
Chromosome: 18
DNA: genomic
Digest: Complete
Insert size range: 2.0 kb average
Genome Equivalent: 72.0
Coverage: Clones for "complete" coverage: 12000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Good resolution and well-isolated #18 peak. Purity estimated by banding: 97%.
Of 20 probes selected at random, 6 inserts were smaller than 300 bp, 11 contained repetitive sequences, and 3 contained unique sequences. Of the latter 3, all mapped to chromosome 18, and 1 contained 3 HindIII fragments contiguous in the genome.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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