ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.1, 3.4, 1.5; HindIII--4.1, 3.4, 1.5; PstI--5.6, 1.3, 0.66, 0.6, 0.47; SalI--9.6; BamHI--5.2, 3.0.
The insert begins 96 bp upstream from the initiation codon, and includes 2.28 kb of coding sequences and a 2.6 kb sequence 3' to the coding region.
References
Rao K, et al. Transcriptional regulation by iron of the gene for the transferrin receptor. Mol. Cell. Biol. 6: 236-240, 1986. PubMed: 3785148
McClelland A, et al. The human tranferrin receptor gene: genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence. Cell 39: 267-274, 1984. PubMed: 6094009
Kuhn LC, et al. Gene transfer, expression, and molecular cloning of the human transferrin receptor gene. Cell 37: 95-103, 1984. PubMed: 6327061
