| Vector Information |
Size (kb): 5.5539999008178710 DESCRIPTION OF VECTOR:
Intact vector size: 5.554
Type of vector: plasmid
Cloning sites: XbaI SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS416, GALL promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: XhoI...XbaI, ->, 2265-2328
promoter for expression: GALL, <-, 2329-2759
marker(s): ampR, <-, 4006-4936
centromere: CEN6/ARSH4, ->, 5040-5554 Vector: p416 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS416, GALL promoter Marker(s):URA3,ampR Construct size (kb): 5.553999900817871 Features: marker(s): URA3
marker(s): ampR
promoter for expression: GALL
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--5.1, 0.43. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
