| 產品名稱 |
Canid herpesvirus 1 |
| 商品貨號 |
B215360 |
| Classification |
Herpesviridae, Varicellovirus |
| Agent |
Canid herpesvirus 1 |
| Strain |
F 205 (Carmichael) |
| Common Name |
Canine herpesvirus |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C |
| Comments |
Derived by adaptation of primary cell culture product ATCC VR-647 to MDCK cells (ATCC CCL-34). ATCC VR-647 and ATCC VR-1785 have not been compared for sequence or infectivity in primary cell culture and tissue culture. Chloroform/ether sensitive, not known to hemagglutinate, sensitive to pH below 6.5 and heat labile. |
| Effect on Host |
CPE, cell rounding and sloughing |
| Recommended Host |
MDCK (ATCC CCL-34) |
| Growth Conditions |
Temperature: 37° Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-4 days |
| Name of Depositor |
JH Gillespie, New York State Veterinary College, Cornell University |
| Source |
Kidney from 2-3 weeks old puppy |
| References |
Carmichael LE, et al. Clinical and pathologic features of a fatal viral disease of newborn pups. Am. J. Vet. Res. 26: 803-814, 1965. PubMed: 5892835
Carmichael LE, et al. Identification of a cytopathogenic agent infectious for puppies as a Canine herpesvirus. Proc. Soc. Exp. Biol. Med. 120: 644-650, 1965. PubMed: 4221827
|
