| 產品名稱 |
pDG1 |
| 商品貨號 |
B215473 |
| Designations |
pDG1 |
| Depositors |
Allelix Biopharmaceuticals, Inc., G Strachan, Allelix Biopharmaceuticals, Inc. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli HB101 |
| Vector Information |
Size (kb): 12.1999998092651400 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873 Vector: pDG1 (cosmid) Construction: pHC79, pBB116 Marker(s):ampR,argB Construct size (kb): 12.19999980926514 Features: marker(s): ampR, argB
replicon: pMB1 |
| Applications |
contains sequence ornithine carbamoyltransferase integrating vector shuttle vector vector for constructing genomic libraries vector permitting positive selection for integration |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--9.4, 1.5, 1.3; EcoRI/BamHI--6.3, 5.9; PstI--10.2, 2.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. This is an integrative, positive selection vector conferring arginine prototrophy on an auxotrophic host such as Aspergillus niger 350(-)52 (ATCC 20739) (argB-). The argB BamHI/EcoRI fragment contains the following sites (separated by approximate kb): BamHI- 0.8- SalI- 0.4- HindIII- 1.4- SalI- 0.1- HindIII- 0.7- SalI- 0.1- HindIII- 0.7- SalI- 0.7- SalI- 0.5- EcoRI. This was constructed by inserting the 5.0 kb EcoRI/HindIII fragment from pBB116 containing argB into pHC79 cut with EcoRI + HindIII. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 30.0°C |
| References |
Buxton FP, et al. Aspergillus niger transformation system. US Patent 4,885,249 dated Dec 5 1989
Berse B, et al. Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans. Gene 25: 109-117, 1983. PubMed: 6363209
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
