ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following restriction sites (separated by approximate kb): HindIII- 1.0- BamHI- 0.3- HindIII- 3.2- HindIII- 1.9- EcoRI- 0.25- BamHI- 1.1- EcoRI.
Restriction digests of the clone give the following sizes (kb): SmaI/SalI--8.6, 7.5, 2.7; SalI--16.0, 2.9; EcoRV--9.0, 6.2, 2.4, 1.0; XbaI--10.5, 4.1 (doublet).
A 1.3 kb HindIII fragment from this insert is sufficient to complement either a hem2-15 (C41/U1) or a hem2-16 (N28/U2) mutation.
The probe, pG32/T4, has been deposited in Escherichia coli RR1 as ATCC 37659 and in Saccharomyces cerevisiae C41/U1 as ATCC 66314.
Myers AM, et al. Characterization of the yeast HEM2 gene and transcriptional regulation of COX5 and COR1 by heme. J. Biol. Chem. 262: 16822-16829, 1987. PubMed: 2445751
