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in another host, produces protein gonadotropin-releasing hormone receptor

Construct size (kb): 5.559999942779541

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I
Intact vector size: 4.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI PstI EcoRV NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI PstI EcoRV BstXI NotI XhoI SphI
NsiI XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
promoter: CMV, ->
promoter: T7, ->
MCS: HindIII...XbaI, ->
promoter: SP6, <-
terminator: SV40 polyadenylation, ->
replicon: polyoma ori
replicon: SV40
replicon: pMB1
replicon: M13 ori, <-
marker(s): supF, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRHR
Genomic copy number: unique
Gene name: gonadotropin-releasing hormone receptor
Contains complete coding sequence?: Y
Tissue: pituitary
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.56
Cross references: DNA Seq. Acc.: L03380

Insert lengths(kb): 1.559999942779541

Tissue: pituitary

Gene product: gonadotropin-releasing hormone receptor [GRHR]

Target Gene: gonadotropin-releasing hormone receptor

Restriction digest of the clone give the following sizes (kb): EcoRI--4.0, 1.55; BamHI--4.95, 0.6; HindIII--4.3, 0.8, 0.4; PstI--4.65, 0.9; PvuII--2.2, 1.8, 1.55.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.55; HindIII--0.42, 1.26; NdeI--1.06; NcoI--0.53; PstI--0.65; PvuII--0.70; SphI--1.42.

Contains the complete coding sequence (nt 56-1042).

The following primers can be used to amplify an 800 bp DNA fragment contained in the gene: upstream 5'-GCTTGAAGCTCTGTCCTGGGA-3'; downstream 5'-CCTAGGACATAGTAGGG-3'.

When transfected into COS-7 cells, expresses a high-affinity receptor.

Detects a 5.0 kb RNA transcript in human pituitary.

Transcription from the T7 promoter gives the sense strand.