Store medium at 2°C to 8°C in the dark when not in use.
Applications
General culture conditions to maintain undifferentiated,
pluripotent mouse ES cells are now well established, and
incremental improvements, such as the development of
Mouse ES Cell Basal Medium, are key to the ongoing
success of stem cell research.
Mouse ES Cell Basal Medium is a sterile liquid tissue culture
medium containing phenol red that has been optimized to
support the undifferentiated self-renewal cycle related to
mouse embryonic stem cell growth.
The medium contains a stable glutamine dipeptide, providing
a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell
Qualified Fetal Bovine Serum (FBS) or a serum replacement
designed for mouse ES cells.
This medium can support the growth of mouse ES cells on
either a mitotically inactivated layer of mouse embryonic
fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Quality Control Specifications
Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also checked for its ability to support the growth of several different cell lines using both sequential subcultures and plating efficiency. Additional test results are listed in the Certificate of Analysis.
