Total size (kb): 4.335 Excise insert: BamHI Vector: pBluescript Type of vector: Phagemid Vector size (kb): 2.96 Insert site(s): BamHI Markers: ampR
Insert
Insert size (kb): 1.377
Source: Human DNA: genomic Gene symbol: DM Gene name: dystrophia myotonica Genbank accession: L00727 (see above) Contains complete coding sequence ?: N Chromosome location: 19 q13.3 Insert ends: BamHI
Insert Size (kb)
1.377
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
In Escherichia coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.4; HindIII--4.4; SmaI--3.8, 0.46.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.01, 1.20; NarI--0.85; SmaI--0.93.
The GCT triplet expansion characteristic of myotonic muscular dystrophy can also be detected using this probe with BanI- or TaqI-digested genomic DNA.
References
Fu YH, et al. An unstable triplet repeat in a gene related to Myotonic Muscular Dystrophy. Science 255: 1256-1258, 1992. PubMed: 1546326
