Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 14.0, 11.0; SalI--21.0, 11.0, 9.0, 6.6; XhoI--23.0, 14.0, 11.0. Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (with selection). Permits construction using partial filling-in. The restriction sites to be used for conversion to phagemid are: SalI ApaI RsrII NheI. Cloning capacity: 18.9 kb. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--2.6 (longer arm), 1.9. The order of the major features in this plasmid is: arm - SalI/NheI - ampR - M13 ori - SacII/MCS/NaeI - stuffer - NaeI/MCS/XbaI - pMB1 ori - NheI/SalI - arm. |
