contains easily purifiable cassette(s) for construction
vector permitting production of single-stranded DNA
Comments
Contains an easily isolated f1 intergenic region for conversion of any plasmid to an ssDNA vector (use SalI + AccI + HincII).
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795
