| 產品名稱 |
YHX133L |
| 商品貨號 |
B216521 |
| Designations |
YHX133L |
| Species |
Homo sapiens, human |
| Depositors |
ER McCabe |
| Vector |
Construct size (kb): 3.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: SmaI
Vector end: SmaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DXS1033
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: X; Localization: X p21
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references: Insert lengths(kb): 0.6000000238418579 Gene product: DNA Segment, single copy [DXS1033] |
| Insert Size (kb) |
0.600 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.9, 0.6; PstI/SacI--2.9, 0.65; BglI--2.2, 1.3; HindIII--3.5; PstI--3.5; PvuII--2.5, 1.0; EcoRI--2.9, 0.6. Maps to YACs 75F7, 332F10, and 406C1 in the adrenal hypoplasia congenita region. |
| References |
Worley KC, et al. Yeast artificial chromosome cloning in the glycerol kinase and adrenal hypoplasia congenita region of Xp21. Genomics 16: 407-416, 1993. PubMed: 8314578
|
