| 產品名稱 |
pENV-3 |
| 商品貨號 |
B217094 |
| Designations |
pENV-3 |
| Species |
human immunodeficiency virus - 1 |
| Depositors |
SM Watanabe |
| Applications |
produces protein env3 |
| Vector |
Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJH11
Intact vector size: 0.520
Type of vector: plasmid
Vector end: HindIII
Vector end: BamHI
Cloning sites: BamHI HindIII
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: trp
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human immunodeficiency virus - 1
Gene symbol: env3
Gene name: env3
Contains complete coding sequence?: U
Strain: BRU
Type of DNA: cDNA
Insert end: BglII
Insert end: HindIII
Insert size (kb): 0.52
Cross references: Insert lengths(kb): 0.5199999809265137 Gene product: env3 [env3] Target Gene: env3 |
| Insert Size (kb) |
0.520 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--3.8, 0.54; HindIII/PstI--3.5, 0.72; EcoRI--4.2. Cloned from HIV 1 BRU. Produces env3. Expression is inducible by tryptophan. Cloned from HIV 1 BRU. Produces env3. Expression is inducible by tryptophan. The construct consists of bp 7178-7698 of HIV env3 gene cloned into expression vector pJH11. Expression is inducible by tryptophan. |
| References |
Watancke SM. Expression and diagnostic use of pENV-3 encoded peptides which are immunologically reactive with antibodies to LAV. US Patent 4,784,941 dated Nov 15 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
