| Karyotype |
45, X, -Y, dup (5), -8, +9, -20, -22, +mar1, +mar2 |
| Comments |
These sequences are known to bind to and inactivate endogenous p53 and Rb proteins respectively.
Southern blot analysis shows that stable integration of the transfected genes occurred.
The cell line resembles morphologically the basal cells of the normal human bronchial epithelium, and is not tumorigenic in athymic nude mice.
The cells are able to form tubules when grown in a basement membrane like matrix, and they retain the ability to undergo terminal squamous differentiation in response to phorbol esters or upon reaching confluence.
Cells are non-viable in DMSO and should be frozen in culture Medium with 10% glycerol.
Original authentication testing at ATCC by isoenzymology indicated that this was a human cell line. Recent additional speciation using a mitochondrial cytochrome c oxidase I (CO1) assay has shown that this cell line is cross-contaminated with bovine cells. Additional more sensitive PCR assays of the original token lot have also indicated a low level of bovine cross-contamination in that material as well. |
| References |
Viallet J, et al. Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16. Exp. Cell Res. 212: 36-41, 1994. PubMed: 8174640
Tsao MS, et al. Autocrine growth loop of the epidermal growth factor receptor in normal and immortalized human bronchial epithelial cells. Exp. Cell Res. 223: 268-273, 1996. PubMed: 8601403
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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