Host for amplifying large yeast artificial chromosomes
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain
no
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Genotype
MATa ura3-52 his3-delta200 leu2-delta1 trp1-delta63 lys2-delta202 GAL+
Preceptrol®
no
Mating Type
a
Comments
Host for amplifying large yeast artificial chromosomes
Medium
ATCC® Medium 2318: 1212 plus 20 mg/L uracil, leucine, tryptophan, histidine and lysine
ATCC® Medium 2318: 1212 plus 20 mg/L uracil, leucine, tryptophan, histidine and lysine
ATCC® Medium 1212: Yeast synthetic minimal medium
Growth Conditions
Temperature: 25.0°C
Name of Depositor
Collaborative Research, Inc.
Isolation
Genetic cross of laboratory bakers' yeast strains.
References
Smith DR, et al. Amplification of large artificial chromosomes. Proc. Natl. Acad. Sci. USA 87: 8242-8246, 1990. PubMed: 2236036
