Insert contains internal BamHI and XbaI sites (one each). Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 1.75; HindIII--5.0; PstI--5.0; BamHI--3.4, 1.6; XhoI--uncut; SalI--5.0; NotI--uncut. This probe identifies a muscle-specific mRNa, 25-30 kb in size, encoding human nebulin. |
