Restriction digests of the clone give the following sizes (kb): KpnI--3.0, 0.26; PstI--2.1, 1.2; NotI--2.4, 0.65, 0.2; EcoRI--3.3; EcoRI/HindIII--3.0, 0.3. The insert contains the following restriction sites (nt from the 5' end): KpnI--62; PstI--222. Encodes the first structural domain of HLA-DRB1*03012. |
