| 產品名稱 |
pmIL5-4G |
| 商品貨號 |
B217729 |
| Designations |
pmIL5-4G |
| Species |
Mus musculus, mouse |
| Depositors |
T Yokota |
| Vector |
Construct size (kb): 6.099999904632568 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.629999995231628 Tissue: T-lymphocyte Cl.Ly1+2-/9 cell line Gene product: interleukin 5 (colony-stimulating factor, eosinophil) [Il5] |
| Insert Size (kb) |
1.630 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Contains internal AccI (1), NcoI (2), NdeI (1), and XmnI (1) sites. Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.7; AccI--3.0, 1.5, 1.2; EcoRI--6.0; EcoRI/NdeI--3.1, 2.1, 0.9. The BamHI fragment from pcD(SR)-mIL5-4G containing interleukin 5 sequences was cloned into the BamHI site of pBR322. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Yokota T, et al. Isolation and characterization of lymphokine cDNA clones encoding mouse and human IGA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5. Proc. Natl. Acad. Sci. USA 84: 7388-7392, 1987. PubMed: 2823259
Takashi Yokota, personal communication
|
