| Vector |
Construct size (kb): 14.19999980926514 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR328
Intact vector size: 4.900
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV BalI NcoI NruI PstI PvuI PvuII SalI SphI XmaIII
Polylinker sites:
Construction: pBR327 ori, pBR325 cmL
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR, tetR
promoter: none
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08858 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 q34.1
Tissue: chronic myelogenous leukemia K-562 cell line
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 9.333
Cross references: DNA Seq. Acc.: U07563
Nucleotides 1-9333 of the insert correspond to
nucleotides 8655-17987 of U07563. Insert lengths(kb): 9.333000183105469 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL] Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--14.0; EcoRI--6.2 (doublet), 1.45; HindIII--5.0, 4.4 (doublet); PstI--7.2, 1.9 (doublet), 1.8, 1.05; PvuII--4.0 (doublet), 3.9, 2.0, 0.26. The insert contains part of the intron between exon 1b and exon 1a. |
| References |
Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859
Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185
Bruce A Roe, personal communication
|
