Contains the following restriction sites (with nucleotide position according to the published sequence): BamHI--608, HindIII--645, HindIII--975. Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 0.45; PstI--3.2; XbaI--3.2; HindIII--2.85, 0.35; EcoRI--2.7, 0.5. Includes sequences from approximately nt 590 to nt 1120 of the published sequence, encoding the calcium-binding site and the ATP binding site. Coding sequences extend from nt 50 to nt 2066. Hybridizes to human sequences and was used to map the human gene. Detects human genomic EcoRI fragments of 4.7 and 3.8 kb and HindIII fragments of 4.2, 7.5, and 3.0 kb. Detects mRNA of 8.1, 3.8, and 3.5 kb in bovine brain and 8.6 kb in rat brain. |
