| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.8, 2.6; EcoRI--5.4; HindIII--2.9, 2.5; KpnI--5.4; SalI--5.4. The insert includes the following restriction sites (approximate kb from the 5' end): PvuII--0.12, 0.76, 1.38, 1.45, 2.23; ApaI--0.78; PstI--0.88, 1.40, 1.78, 2.3; SacI--0.89, 1.39; BglII--1.61; HindIII--2.48. A HindIII/SphI digestion produces fragments of 1.630 kb (without tails) and 0.855 kb (with a GC tail). The XbaI site at nt 4730 of the reported sequence does not cut. Extends from nt 2641 to 5296 of the reported sequence, encoding part of domains I and III and all of domains II and alpha. |
| References |
Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975
Sasaki M, et al. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc. Natl. Acad. Sci. USA 84: 935-939, 1987. PubMed: 3493487
Ilse Oberbaumer, personal communication
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