| 產(chǎn)品名稱 | BJ-5ta |
|---|---|
| 商品貨號 | B218466 |
| Organism | Homo sapiens, human |
| Tissue | Foreskin |
| Cell Type | Fibroblast immortalized with hTERT |
| Product Format | frozen |
| Morphology | Fibroblast-like |
| Culture Properties | Adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Normal |
| Age | Neonatal |
| Gender | Male |
| Storage Conditions | Liquid nitrogen vapor phase |
| Karyotype | This is a diploid human cell line of male origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes, X and Y are both karyotypically normal. |
| Images |  |
| Derivation | The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332]. |
| Antigen Expression | Antigen expression: Positive for fibroblast surface protein; Homo sapiens, expressed (fibroblast surface protein (FSP) was assayed by flow cytometry.). Negative for the pan-cytokeratin epithelial marker; Homo sapiens (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody). |
| Complete Growth Medium | A 4:1 mixture of Dulbecco's medium and Medium 199 with supplements as follows : 4 parts of Dulbecco's Modified Eagle's Medium containing 4 mM L-glutamine, 4.5 g/L glucose and 1.5 g/L sodium bicarbonate 1 part of Medium 199 Supplemented with: 0.01 mg/ml hygromycin B 10% fetal bovine serum |
| Subculturing | Volumes are given for a 75 cm2 flask. Recommended use of Corning® T-75 flasks (catalog #430641). Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 twice weekly
Medium Renewal: every 2 to 3 days
Note: Subculture when cell concentration reaches between 8 X 103 and 1 X 104 cells/cm2.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation | Freeze medium: culture medium, 30%; fetal bovine serum, 60%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,9 D16S539: 9,13 D5S818: 12 D7S820: 11,12 THO1: 7,8 TPOX: 10,11 vWA: 16,18 |
| Population Doubling Level (PDL) | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor | Geron Corporation |
| Deposited As | Homo sapiens |
| References | Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802 Aiastui A, et al. Salmonella enterica serovar typhimurium invades fibroblasts by multiple routes differing from the entry into epithelial cells. Infect Immun 78(6):2700-13, 2010. PubMed: 20368348 Janeckova L, et al. HIC1?Tumor?Suppressor?Loss?Potentiates?TLR2/NF-κB?Signaling?and?Promotes?Tissue?Damage-Associated?Tumorigenesis. Mol Cancer Res?13(7):1139-48, 2015. PubMed: 25934696 Amadori S, et al. Effect of sterilization and crosslinking on gelatin films. J Mater Sci Mater Med 26(2):69, 2015. PubMed: 25631265 La Gatta A, et al. Hyaluronan dermal fillers via crosslinking with 1,4-butandiol diglycidyl ether: Exploitation of heterogeneous reaction conditions. J Biomed Mater Res B Appl Biomater 104(1):9-18, 2016. PubMed: 25611588 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
