A 3' PvuII/EcoRI fragment (nucleotides 930 - 1420) has been used as a specific probe.
Vector
Construct size (kb): 4.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Tissue: fetal skeletal muscle
Gene product: myogenin (myogenic factor 4) [MYF4]
Insert Size (kb)
1.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains XhoII, PstI, and PvuII sites. A 3' PvuII/EcoRI fragment (nucleotides 930 - 1420) has been used as a specific probe.
Restriction digests of the clone give the following sizes (kb): PstI--4.0, 0.45; BamHI--4.5; EcoRI--3.3, 1.3; SmaI--4.5; SalI--4.5.
Myf-4 is the myogenin homolog. This clone does not include the complete coding sequences, but extends from nucleotides 170 - 1420 and encodes the Myf homology motif (290 - 462). Coding sequences are 53 - 790.
This clone detects a genomic EcoRI fragment of 6.5 kb and an mRNA of about 1.9 kb.
References
Braun T, et al. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 8: 701-709, 1989. PubMed: 2721498
Braun T, et al. Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products. EMBO J. 8: 3617-3625, 1989. PubMed: 2583111
