Reported to contain genomic EcoRI/HindIII fragments of the following sizes (kb): 5.62, 3.24, 3.15, 2.64, 1.77, 1.11, 1.03, 0.94, 0.76, 0.55, 0.5. Also contains fragments of 14.94 (CUP1?) and 8.4 (vector-associated). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 209622-240071 of the sequence of chromosome VIII. RPP1 is an essential gene encoding a 32.2 kD ortholog of the human scleroderma autoimmune antigen Rpp30. The gene is contained in a 1.4 kb BamHI/XbaI fragment. |
