| Vector |
Construct size (kb): 4.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S8933E
Gene name: DNA Segment, expressed
Contains complete coding sequence?: U
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5' end: Modification: HindIII
Insert 3' end: Modification: NotI
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: T03725 Insert lengths(kb): 1.5 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: DNA Segment, expressed [D0S8933E] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; ClaI--4.6; HindIII/NotI-- 3.1, 0.9, 0.6; PvuII--2.8, 1.3, 0.31; ScaI--4.6; EcoRI--4.6. Derived from a directionally cloned library with an average insert size of 1.7 kb. Inserts can be amplified with the M13 universal (forward) and reverse primers. The primers can also be used to generate sequence from the 3' or 5' ends, respectively. |
| References |
Khan AS, et al. Single pass sequencing and physical and genetic mapping of human brain cDNAs. Nat. Genet. 2: 180-185, 1992. PubMed: 1345165
Soares MB, et al. Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. USA 91: 9228-9232, 1994. PubMed: 7937745
Marcelo B Soares, personal communication
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