The insert contains the following sites separated by (bp) (approx): 5' PstI- 231- BamHI- 210- EcoRI- 218- NdeI- 481- 3'. Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.0, 0.8, 0.45; PstI/XbaI--7.0, 1.3; EcoRI--7.5, 0.8; HindIII--8.4; BamHI--7.4, 1.0. This is the nonsense strand of the 512 to 1642 bp fragment of the CSF gene. The insert is in a reversed orientation in the vector. |
