| Vector Information |
Size (kb): 6.4889998435974120 DESCRIPTION OF VECTOR:
Intact vector size: 6.489
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS423, GAL1 promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): HIS3, ->, 200-1395
terminator: CYC1, ->, 2077-2337
MCS: XhoI...SpeI, ->, 2337-2394
promoter for expression: GAL1, <-, 2401-2861
marker(s): ampR, <-, 4012-4942
replicon: 2 micron, ->, 5042-6382 Vector: p423 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS423, GAL1 promoter Marker(s):ampR,HIS3 Construct size (kb): 6.488999843597412 Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GAL1
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.45; KpnI--5.5, 1.0; PstI--5.3, 1.2. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
