| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5, 2.7; PstI--3.1, 3.1; HindIII/EcoRI--2.7, 1.9, 1.7; HindIII--4.3, 1.9. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--1.9; PstI--3.2; XbaI--3.2. The complete probe detects genomic EcoRI fragments of the following sizes (kb): human--15, 6.5, 3.7, 3.0, 1.8, 1.0, 0.3; mouse--16, 9.0, 4.0, 3.8, 1.0; Chinese hamster--7.5, 7.3, 2.0. The 5' 1.9 kb EcoRI/HindIII fragment from the insert, that contains the 3' coding region of the transcript, detects human genomic PstI fragments of the following sites (kb): 19, 6.8, 5.0, 3.7, 3.0, and 1.8. The 3' 1.6 kb HindIII/EcoRI fragment from the insert, that contains 3' untranslated sequences, detects genomic PstI fragments of the following sizes (kb): human--1.8; mouse--15; rat--7.0; hamster--7.0. Detects a transcript of 13 kb that is expressed ubiquitously. |
| References |
Love DR, et al. An autosomal transcript in skeletal muscle with homology to dystrophin. Nature 339: 55-58, 1989. PubMed: 2541343
Love DR, et al. Tissue distribution of the dystrophin-related gene product and expression in the mdx and dy mouse. Proc. Natl. Acad. Sci. USA 88: 3243-3247, 1991. PubMed: 2014247
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