| 產品名稱 |
yRM2003 [HTY275] |
| 商品貨號 |
B221663 |
| Designations |
yRM2003 [HTY275] |
| Species |
Homo sapiens, human |
| Depositors |
HC Riethman |
| Vector |
Construct size (kb): 130.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTYAC1
Intact vector size: 9.000
Type of vector: YAC
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
restriction site: EcoRI
other: telomere, Tetrahymena, <-
marker(s): URA3
centromere: CEN4
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D2S446
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 2; Localization: 2 q37
Genome: Homo sapiens
Gene symbol: D2Z4
Gene name: DNA Segment, repetitive (telomere)
Contains complete coding sequence?: U
Chromosome: 2; Localization: 2 q37-qter
Tissue: leukocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 120.0
Cross references: Insert lengths(kb): 120.0 Tissue: leukocyte Gene product: DNA Segment, repetitive (telomere) [D2Z4] |
| Insert Size (kb) |
120.0 |
| Media |
AHC- (AHC plus 40 mg/L tryptophan)
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen |
| Comments |
Contains at least two CpG islands in the duplicated subtelomeric DNA approximately 100 kb from the telomere. This sequence is also available in the cosmid 2003 cosmid 1 (ATCC 99432). The insert of yRM2003 (ATCC 99419) is contained within the insert of yRM2112 (ATCC 99418). |
| References |
Macina RA, et al. Sequence organization of the human chromosome 2q telomere. Hum. Mol. Genet. 3: 1847-1853, 1994. PubMed: 7545974
Riethman HC, et al. Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector. Proc. Natl. Acad. Sci. USA 86: 6240-6244, 1989. PubMed: 2668959
|
