| Vector |
Construct size (kb): 4.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: D0S143E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens cytochrome P450, subfamily XIB, polypeptide 1
(steroid 11-beta-hydroxylase)
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M85450; EST: EST01966; Primer: M13 Forward;
THC id: THC91329
DNA Seq. Acc.: T04959; EST: EST02847; Primer: M13-21; THC
id: THC91329
DNA Seq. Acc.: T04960; EST: EST02848; Primer: M13 Reverse Insert lengths(kb): 1.799999952316284 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: cytochrome P450, subfamily XIB, polypeptide 1 (steroid 11-beta-hydroxylase) [CYP11B] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.8; PstI--4.8; HindIII--4.8. EST01966 matches GenBank accession HUMCYPXIB with 67.35% identity over the sequence range: 113-256. EST01966 contains a Line-1 repeat element. This sequence record is for human cytochrome P450, subfamily XIB, polypeptide 1 (steroid 11-beta-hydroxylase) (CYP11B1). |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
J C Venter, personal communication
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